Open-Source Fluidics IAP 2015
David Sun Kong, Ph.D., Technical Staff - Bioengineering Systems & Technologies MIT Lincoln Laboratory
MIT IAP 2015 Graduate Course 20.S591
“Open-Source Fluidics for Synthetic Biology: Prototyping Microbial Communities”
The human gut is an astonishing ecosystem of trillions of micro-organisms that live in a remarkable harmony with us, the human host. In this intensive graduate course, students developed 3D design and fabrication skills and constructed fluidic "artificial guts." Students utilized these structures to culture a variety of gut-based micro-organisms, both in mono- and co-cultures, to prototype and construct from scratch their own microbial communities.
Class 1 | Introduction |
Course overview, Talks "Synthetic Biology," Dr. Peter Carr (MIT Lincoln Laboratory); "Microfluidics," Dr. Todd Thorsen (MIT Lincoln Laboratory) |
|
Class 2 | 3D Design |
3D design workshop by Brian Chan, Talk "3D Printed Hardware for DNA Assembly," Will Patrick (MIT Media Lab) |
|
Class 3 | Fluidics & Fabrication |
High-throughput microfluidics and sequencing the "dark matter" of microbes. 3D stereolithography printing workshop by Formlabs. Talk "Microfluidics for Microbial Genomics," Professor Paul Blainey (MIT Broad Institute) |
|
Class 4 | Engineering the Human Microbiota |
Process first prints, biology lab (68-074) safety training. Talk "Making the Microbiota - how microbes colonize the gut," Sean Kearney (MIT Human Microbiome Center) Talk "Engineering the human microbiome: Designing personal analytics technology," Mariana Matus and Yaniv Turgeman (MIT Human Microbiome Center). |
|
Class 5 | Fecal Matter Transplants |
Process prints, start microbial cell culture, Talk "Open Biome," Mark Smith (President & Co-Founder, www.openbiome.org) |
|
Class 6 | Open-Source Devices |
Talk: "Little Devices," Jose Gomez-Marquez (Principal, Little Devices) |
|
Class 7 | Programmable 3D Fluidics |
Talk: "Multi-material 3D Valves," Steven Keating & Will Patrick (MIT Media Lab) |
|
Class 8 | Lab Work |
Class 9 | Final Presentations |
Dr. David Sun Kong (Group 48, MITLL) with students and mentors at MITLL Beaver Works (IAP 2015) |
|